For up-to-the-minute information, the Texas Department of Transportation has a wildflower hotline at 1-800-452-9292.
These routes are longtime favorites confirmed by multiple sources, including wildflowersightings.org.
Wildflower Corridors
I-35 between Dallas-Fort Worth and South Texas
I-10 between San Antonio and Brookshire
Central Texas
Blanco and Burnet counties
A. U.S. 281 between Johnson City and Marble Falls
Blanco, Comal and Bexar counties
B. U.S. 281 between Blanco and San Antonio
Gillespie County
C. Willow City Loop (near TX 16 off FM 1323)
D. Lyndon B. Johnson State Park & Historic Site, U.S. 290 east of Stonewall
Llano County
E. Around Lake Buchanan and Inks Lake, along FM 1431
Mason County
F. Mason County Loop (from U.S. 87 southeast of Mason, take RR 783 south to Threadgill Creek Road, turn left, and go back to U.S. 87)
Travis and Bastrop counties
G. U.S. 290 between Manor and Paige
H. TX 71 between Austin and Bastrop
Williamson and Burnet counties
I. TX 29 between Georgetown and Burnet
East Texas
Angelina and Nacogdoches counties
J. U.S. 59 between Lufkin and Nacogdoches
Bowie, Morris and Titus counties
K. I-30 between Texarkana and Mount Pleasant
Houston, Trinity and Polk counties
L. U.S. 287 between Crockett and Corrigan
Marion, Cass and Morris counties
M. TX 49 between Jefferson and Daingerfield
Montgomery County
N. I-45 and TX 105 near Lake Conroe
Nacogdoches and Crockett counties
O. TX 7 between Nacogdoches and Crockett
Tyler County
P. Around Woodville
North Central Texas
Bastrop, Lee and Burleson counties
Q. TX 21 between Paige and Caldwell
Dallas County
R. I-45 just south of Dallas, near Hutchins
Fayette County
S. I-10 between Schulenburg and Flatonia
Fayette and Austin counties
T. TX 159 between La Grange and Bellville
Waller and Washington counties
U. U.S. 290 between Hempstead and Brenham
Washington County
V. Most anywhere in Washington County
West Texas
Brewster County
W. Big Bend National Park, around Study Butte and Panther Junction